When assessing proteins in a sample, enzyme-linked immunosorbent assays (ELISAs) quantitate antibody binding to a target but fail to provide protein size information. As such, researchers may miss important details, including the presence of isoforms, degradation products, and aggregates, that would be apparent on a western blot. While traditional western blot assays involve multiple manual steps, an automated capillary electrophoresis immunoassay platform offers scientists the best of both worlds—sensitivity and accuracy comparable to ELISAs plus molecular weight information—without messy blots, gels, and transfer and detection equipment.

Download this review from Bio-Techne to learn how researchers use Simple Western™ for accurate and reproducible protein analysis during basic research and therapeutic development.

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