A photo of a sample storage box sitting on metal racks in a liquid nitrogen-cooled freezer.
Article

Best Practices for Freezing Cell Cultures

Learn how to store animal cells long-term.

Share

Cryopreservation is a crucial process for ensuring the long-term viability and genetic integrity of animal cell cultures. Inconsistent freezing methods and contamination risks can undermine experimental accuracy, disrupt workflows, and potentially result in the permanent loss of valuable cell lines. As cryogenic storage plays an increasingly vital role in biomedical research, mastering both the theory and practice of cell freezing is essential for obtaining reliable and reproducible results.

Download this guide to learn about 

  • Strategies for preventing cell damage during freezing and thawing
  • Key considerations for choosing cryoprotective agents and storage vessels
  • Techniques for diagnosing and resolving cryogenic storage problems
  • Essential steps in safeguarding valuable cultures in a cell repository

Sponsored by

  • Corning logo

Top Image Credit:

©iStock, dit:zmeel

February 2026

A Stubborn Gene, a Failed Experiment, and a New Path

When experiments refuse to cooperate, you try again and again. For Rafael Najmanovich, the setbacks ultimately pushed him in a new direction.

View this Issue
Human-Relevant In Vitro Models Enable Predictive Drug Discovery

Advancing Drug Discovery with Complex Human In Vitro Models

Stemcell Technologies
Redefining Immunology Through Advanced Technologies

Redefining Immunology Through Advanced Technologies

Ensuring Regulatory Compliance in AAV Manufacturing with Analytical Ultracentrifugation

Ensuring Regulatory Compliance in AAV Manufacturing with Analytical Ultracentrifugation

Beckman Coulter Logo
Conceptual multicolored vector image of cancer research, depicting various biomedical approaches to cancer therapy

Maximizing Cancer Research Model Systems

bioxcell

Products

Sino Biological Logo

Sino Biological Pioneers Life Sciences Innovation with High-Quality Bioreagents on Inside Business Today with Bill and Guiliana Rancic

Sino Biological Logo

Sino Biological Expands Research Reagent Portfolio to Support Global Nipah Virus Vaccine and Diagnostic Development

Beckman Coulter

Beckman Coulter Life Sciences Partners with Automata to Accelerate AI-Ready Laboratory Automation

Refeyn logo

Refeyn named in the Sunday Times 100 Tech list of the UK’s fastest-growing technology companies