A photo of a sample storage box sitting on metal racks in a liquid nitrogen-cooled freezer.
Article

Best Practices for Freezing Cell Cultures

Learn how to store animal cells long-term.

Share

Cryopreservation is a crucial process for ensuring the long-term viability and genetic integrity of animal cell cultures. Inconsistent freezing methods and contamination risks can undermine experimental accuracy, disrupt workflows, and potentially result in the permanent loss of valuable cell lines. As cryogenic storage plays an increasingly vital role in biomedical research, mastering both the theory and practice of cell freezing is essential for obtaining reliable and reproducible results.

Download this guide to learn about 

  • Strategies for preventing cell damage during freezing and thawing
  • Key considerations for choosing cryoprotective agents and storage vessels
  • Techniques for diagnosing and resolving cryogenic storage problems
  • Essential steps in safeguarding valuable cultures in a cell repository

Sponsored by

  • Corning logo

Top Image Credit:

©iStock, dit:zmeel

Image of a woman with her hands across her stomach. She has a look of discomfort on her face. There is a blown up image of her stomach next to her and it has colorful butterflies and gut bacteria all swarming within the gut.
November 2025, Issue 1

Why Do We Feel Butterflies in the Stomach?

These fluttering sensations are the brain’s reaction to certain emotions, which can be amplified or soothed by the gut’s own “bugs".

View this Issue
Olga Anczukow and Ryan Englander discuss how transcriptome splicing affects immune system function in lung cancer.

Long-Read RNA Sequencing Reveals a Regulatory Role for Splicing in Immunotherapy Responses

Pacific Biosciences logo
Research Roundtable: The Evolving World of Spatial Biology

Research Roundtable: The Evolving World of Spatial Biology

Conceptual cartoon image of gene editing technology

Exploring the State of the Art in Gene Editing Techniques

Bio-Rad
Conceptual image of a doctor holding a brain puzzle, representing Alzheimer's disease diagnosis.

Simplifying Early Alzheimer’s Disease Diagnosis with Blood Testing

fujirebio logo

Products

Eppendorf Logo

Research on rewiring neural circuit in fruit flies wins 2025 Eppendorf & Science Prize

Evident Logo

EVIDENT's New FLUOVIEW FV5000 Redefines the Boundaries of Confocal and Multiphoton Imaging

Evident Logo

EVIDENT Launches Sixth Annual Image of the Year Contest

10x Genomics Logo

10x Genomics Launches the Next Generation of Chromium Flex to Empower Scientists to Massively Scale Single Cell Research