Biochemistry

T.A. Rooney, E.J. Sass, A.P. Thomas, "Agonist-induced cytosolic calcium oscillations originate from a specific locus in single hepatocytes," Journal of BiologicalChemistry, 265:10792-96, 1990. Andrew Thomas (Thomas Jefferson University, Philadelphia): "Recent improvements in digital imaging microscopy have resulted in revolutionary advances in our understanding of intracellular calcium homeostasis. These techniques have demonstrated that activation of receptors that act via the second messenger


Register for free to listen to this article
Listen with Speechify
0:00
1:00
Share

T.A. Rooney, E.J. Sass, A.P. Thomas, "Agonist-induced cytosolic calcium oscillations originate from a specific locus in single hepatocytes," Journal of BiologicalChemistry, 265:10792-96, 1990.

Andrew Thomas (Thomas Jefferson University, Philadelphia): "Recent improvements in digital imaging microscopy have resulted in revolutionary advances in our understanding of intracellular calcium homeostasis. These techniques have demonstrated that activation of receptors that act via the second messenger inositol 1,4,5-trisphosphate can result in increases in intracellular calcium ([Ca2+]i), which are encoded in the form of [Ca2+]i oscillations in individual cells. In addition to the temporal organization provided by [Ca2+]i oscillations, we demonstrated a further level of organization of Ca2+ signaling in the spatial domain within individual primary cultured hepatocytes.

"Hormone-induced [Ca2+]i oscillations do not occur synchronously throughout the cell, but they originate as waves of Ca2+ from a specific region adjacent to the cell membrane and then they are self-propagated through the cell.

"Furthermore, the oscillatory [Ca2+]i ...

Interested in reading more?

Become a Member of

The Scientist Logo
Receive full access to digital editions of The Scientist, as well as TS Digest, feature stories, more than 35 years of archives, and much more!
Already a member? Login Here
3D illustration of a gold lipid nanoparticle with pink nucleic acid inside of it. Purple and teal spikes stick out from the lipid bilayer representing polyethylene glycol.
February 2025, Issue 1

A Nanoparticle Delivery System for Gene Therapy

A reimagined lipid vehicle for nucleic acids could overcome the limitations of current vectors.

View this Issue
Enhancing Therapeutic Antibody Discovery with Cross-Platform Workflows

Enhancing Therapeutic Antibody Discovery with Cross-Platform Workflows

sartorius logo
Considerations for Cell-Based Assays in Immuno-Oncology Research

Considerations for Cell-Based Assays in Immuno-Oncology Research

Lonza
An illustration of animal and tree silhouettes.

From Water Bears to Grizzly Bears: Unusual Animal Models

Taconic Biosciences
Sex Differences in Neurological Research

Sex Differences in Neurological Research

bit.bio logo

Products

Tecan Logo

Tecan introduces Veya: bringing digital, scalable automation to labs worldwide

Explore a Concise Guide to Optimizing Viral Transduction

A Visual Guide to Lentiviral Gene Delivery

Takara Bio
Inventia Life Science

Inventia Life Science Launches RASTRUM™ Allegro to Revolutionize High-Throughput 3D Cell Culture for Drug Discovery and Disease Research

An illustration of differently shaped viruses.

Detecting Novel Viruses Using a Comprehensive Enrichment Panel

Twist Bio