Live cell imaging allows researchers to investigate dynamic processes by visually recording cells in their living state, rather than obtaining endpoint images that often contain fixation artifacts. With the help of multiplex microscopy, scientists can observe several targets within cells, tissues, or whole organisms, but there are technical aspects of multicolor live cell experiments that researchers must consider.

Download this ebook from Leica Microsystems to explore key considerations for setting up successful multiplexed live cell imaging experiments, such as

  • Selecting the right fluorophores
  • Physiological conditions and image acquisition 
  • Fast and gentle technologies for multicolor fluorescent imaging

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