Check It Out

HatCheck Graph Expensive custom peptide synthesis, hard-to-find reagents, messy filter-binding assays, and liters of radioactive waste: These are what researchers can expect when assaying novel proteins for histone acetyl-transferase (HAT) activity. And yet a growing understanding of the role of chromatin remodeling in transcriptional regulation has made it necessary for more and more scientists to perform these tedious assays. To help alleviate some of these woes, Pierce Chemical Compan

| 2 min read

Register for free to listen to this article
Listen with Speechify
0:00
2:00
Share

HatCheck Graph

Expensive custom peptide synthesis, hard-to-find reagents, messy filter-binding assays, and liters of radioactive waste: These are what researchers can expect when assaying novel proteins for histone acetyl-transferase (HAT) activity. And yet a growing understanding of the role of chromatin remodeling in transcriptional regulation has made it necessary for more and more scientists to perform these tedious assays.

To help alleviate some of these woes, Pierce Chemical Company of Rockford, Ill., has recently introduced the HAT-Check Assay Kit. For this kit, Pierce has gathered together all of the materials needed for the assay, streamlined the procedure, and significantly reduced the amount of radioactive waste generated.

Provided with the assay is a 23-amino acid peptide substrate, derived from the acetylation target sequence of the histone H4 subunit. The researcher simply combines this substrate with reaction buffer and the protein to be tested for HAT activity, along with a small amount ...

Interested in reading more?

Become a Member of

The Scientist Logo
Receive full access to digital editions of The Scientist, as well as TS Digest, feature stories, more than 35 years of archives, and much more!
Already a member? Login Here

Meet the Author

  • Jeanne Mcadara

    This person does not yet have a bio.

Published In

Share
3D illustration of a gold lipid nanoparticle with pink nucleic acid inside of it. Purple and teal spikes stick out from the lipid bilayer representing polyethylene glycol.
February 2025, Issue 1

A Nanoparticle Delivery System for Gene Therapy

A reimagined lipid vehicle for nucleic acids could overcome the limitations of current vectors.

View this Issue
Enhancing Therapeutic Antibody Discovery with Cross-Platform Workflows

Enhancing Therapeutic Antibody Discovery with Cross-Platform Workflows

sartorius logo
Considerations for Cell-Based Assays in Immuno-Oncology Research

Considerations for Cell-Based Assays in Immuno-Oncology Research

Lonza
An illustration of animal and tree silhouettes.

From Water Bears to Grizzly Bears: Unusual Animal Models

Taconic Biosciences
Sex Differences in Neurological Research

Sex Differences in Neurological Research

bit.bio logo

Products

Photo of a researcher overseeing large scale production processes in a laboratory.

Scaling Lentiviral Vector Manufacturing for Optimal Productivity

Thermo Fisher Logo
Collage-style urban graphic of wastewater surveillance and treatment

Putting Pathogens to the Test with Wastewater Surveillance

An illustration of an mRNA molecule in front of a multicolored background.

Generating High-Quality mRNA for In Vivo Delivery with lipid nanoparticles

Thermo Fisher Logo
Tecan Logo

Tecan introduces Veya: bringing digital, scalable automation to labs worldwide