Single-stranded DNA is mixed with a primer and split into four aliquots, each containing DNA polymerase, four deoxyribonucleotide triphos-phates and a replication terminator. Each reaction proceeds until a replication-terminating nucleotide is added. The mixtures are loaded into separate lanes of a gel and electrophoresis is used to for an illustration of a high-speed DNA sequencer.)
What's happening in the DNA sequencing industry? After all, the human genome sequence is done and dusted. Two big players in the industry have recently undergone major changes: Michael W. Hunkapillar, who developed the technology that made high-throughput sequencing possible, stepped down in August from his role as president of Applied Biosystems. And Amersham was purchased by General Electric this year and rebranded as GE Healthcare. Upstart companies are developing a new wave of technologies to challenge capillary technology, currently the only game in town. For the time being it is business as usual, but ...
