J. Villén et al., "Large-scale phosphorylation analysis of mouse liver," Proc Natl Acad Sci, 104:1488-93, 2007. (Cited in 75 papers)
Steven Gygi and colleagues at the Harvard Medical School combined an array of analytical methods—including phosphopeptide enrichment, high performance mass spectrometry, and an optimized data-sorting algorithm—to generate the first systematic survey of post-translational protein phosphorylations in a single organ, the mouse liver.
Gygi's team discovered more than 5,000 phosphorylation sites across some 2,300 proteins in the liver that had not been previously identified. "It's an order of magnitude higher [than found in earlier studies], so you really have a good overview of what's happening," says Heribert Hirt at the Plant Genomics Research Unit (URGV) in Evry, France.
In general, phosphorylation is much more frequent at the C-terminal region than anywhere else on the protein. Since integral membrane proteins are usually oriented with the C-terminus in the intracellular space, these regions ...
