Formalin-based fixation and paraffin embedding (FFPE) is a common method to preserve clinical samples, and FFPE tumor samples serve as valuable study materials for clinical and translational medicine research. While this process enables a variety of downstream analyses, including histology and immunohistochemistry, obtaining high-quality DNA for whole genome sequencing from FFPE-treated tissues is challenging because formalin fixation can lead to DNA fragmentation.
Download this application note from Agilent to discover how to establish a reproducible workflow to obtain a high-quality whole-exome sequencing library.