Stratagene's Vitality™ hrGFP (green fluorescent protein) mammalian expression vectors allow protein expression and subcellular localization studies similar to those using jellyfish (Aequorea victoria) GFP, with one important difference: Stratagene's hrGFP exhibits less toxicity in many mammalian cells.
Renilla reniformis, an Anthazoan sea pansy related to the jellyfish, produces a 238 amino-acid protein that has broader pH stability, more resistance to solvents, detergents, and proteases, and a 2.5-fold higher extinction coefficient than does the red-shifted Aequorea protein. While it has less than 30 percent homology to the Aequorea protein, the hrGFP chromophore is similarly derived from a post-translational cyclization of a tripeptide and also exhibits an emission maximum at just over 500 nm. For expression studies and vector construction, La Jolla-based Stratagene constructed a synthetic gene that uses codons preferred in highly-expressed human genes.
Stratagene's Vitality hrGFP vectors Toxicity from Aequorea GFP leads to problems in many cell lines, as shown ...
