Today, flow cytometry users can choose between hundreds of different fluorochromes. On the one hand, this gives them unprecedented flexibility. On the other hand, learning the properties of each fluorochrome to ensure minimal spillover can be daunting. Often, scientists will default to familiar fluorochromes, but this can limit the scope of their experiments and the quality of their data.
Download this guide from BD Biosciences to learn which fluorochromes pair best with which antigens, and discover how to simplify panel design without compromising data resolution or quality.