Site Saturation Variant Libraries (SSVLs) are synthetic libraries for protein engineering. SSVLs are designed alternatives to libraries generated by random error-prone PCR (epPCR), a conventional technique that suffers from amplification bias, incomplete variant representation, and premature stop codons. Scientists use SSVLs to overcome the limitations of epPCR for protein engineering and directed evolution experiments.
Download this application note from Twist Bioscience to learn how scientists enhanced G protein-coupled receptor engineering with SSVLs.