Cell-free DNA (cfDNA) can act as a powerful biomarker for cancer research, from disease onset to therapy selection and minimal residual disease (MRD) measurement. Next-generation sequencing (NGS) has surfaced as a valuable tool to characterize cfDNA. The first step in this process is extracting cfDNA and preparing a sample library. However, cfDNA library preparation must reliably capture and convert fragmented DNA within the sample, as attaining low variant detection thresholds and high variant calling confidence demands high-performance NGS libraries and targeted sequencing protocols.
Download this poster from Twist Bioscience to discover a workflow for cfDNA library preparation that includes an optimized target enrichment protocol to maximize duplex, on-target, sequenceable sample molecule conversion.
For Research Use Only. Not for Use in Diagnostic Procedures.
