Cell-free DNA (cfDNA) contains potential biomarkers for cancer detection, diagnosis, and progression. Scientists use next-generation sequencing (NGS) to characterize cfDNA. The first step in this process is extracting cfDNA and preparing a sample library. However, cfDNA library preparation must reliably capture and convert all the fragmented DNA within the sample, as attaining low variant detection thresholds and variant calling confidence demands high-performance NGS libraries and targeted sequencing protocols. 

Download this poster from Twist Bioscience to discover a workflow for cfDNA library preparation that includes an optimized target enrichment protocol to maximize duplex, on-target, sequenceable sample molecule conversion.

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