Although some mention will be made of prepacked media, the major focus will be on bulk media researchers can use to pack their own columns. In addition, columns and media for analytical research (molecular size determination, quantitation, and/or small molecule identification), bioprocessing, and high pressure liquid chromatography will not be emphasized.
Most, if not all, protein purification schemes involve at least one liquid chromatographic step. The number of steps, matrices utilized, and order depend largely upon the physicochemical properties of the protein of interest and the biological source of the protein. The first chromatographic step in most purification schemes is size exclusion chromatography (SEC); because of this and the simplicity of the technique, it is perhaps the most widely utilized chromatographic protocol. Protein fractionation (separation) is achieved by passage through a matrix composed of inert beads with defined porosity. Proteins in the fractionation range of the matrix are momentarily caught ...
