Liquid chrom-atography is to the protein re-searcher what the hammer is to the carpenter. Liquid chromatographic techniques comprise a major portion of the repertoire of protocols the protein researcher can call upon to purify and analyze proteins. Modern liquid chromatography has come a long way since its infancy-when early matrices were capable of providing only crude separations-to modern matrices and technologies that can accomplish the purification of a protein to homogeneity in a single chromatographic step. A list of representative purification applications for matrices available today is presented in the Application Guide (see page 19). The term chromatography originates from Mikhail Tswett's work on plant pigments (chromophores) in the early 1900s. But modern liquid chromatography had its beginnings in the late 1950s with the introduction of cellulose-based ion exchangers (E.A. Petersen, H.A. Sorbet, J. Am. Chem. Soc., 78:751-755, 1956) and cross-linked dextran size exclusion media (J. Porath, P....
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