Fluorescence microscopy of calcein-labeled cells. The Influx™ cell-loading reagent was used to load RBL cells with calcein. The image was acquired using a fluorescence microscope equipped with a cooled CCD camera and a bandpass filter set appropriately for fluorescein. The micrograph was reprinted with permission from Molecular Probes, Inc.
Do you need to "light up" your cells with a colorful tracer or study the effects of loading cells with a given macromolecule? Representatives at Molecular Probes, Eugene, Ore., say that many of their customers asked for a simple large-scale method for cell loading. The Influx pinocytic cell-loading reagent was developed in response to these requests for an easy way to deliver water-soluble compounds to the cytosol of cultured cells. Tailor-made for convenience, this product is used in a method that harnesses the eukaryotic cell's endocytic pathway.

In endocytosis, cells use an elaborate internal membrane system to ingest macromolecules and deliver...

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