Faster, cheaper, cleaner. All of these words describe Genomyx's latest addition to its line of products for differential display analysis of gene expression. The new Fluorescent Differential Display Adaptor Kit (fluoroDD Kit) provides fluorescently labeled primers that can be used with Genomyx's HIEROGLYPHTM mRNA Profile Kits to allow for a rapid and nontoxic alternative to radioactive labeling of differential display products.
Fluorescent Differential Display with fluoroDDTM mRNA Profile System. Paired samples produced from human fetal and adult liver were run after priming with different anchored and arbitrary primers. ARP = arbitrary primers, TMR-AP = TMR-labeled anchored primer, HFL = human fetal liver, HAL = human adult liver While differential display analysis was embraced by many looking for a fast way to identify and isolate differentially expressed gene sequences, numerous technical difficulties hamper its usefulness. Frequently encountered problems include the generation of irreproducible fragment patterns, the inability to resolve fragments adequately, ...
