Manufactured by Biometra (Germany), the Rotaphor utilizes patented Rotating Field Electrophoresis (ROFE) technology to allow separation beyond the limit of conventional horizontal agarose gel electrophoresis. With this method, an electric field between two primary sets of electrodes is stabilized by two sets of secondary electrodes. The electrodes are positioned within a rotor that is rotated above a stationary gel. This format gives users enormous flexibility by choosing vector angles between 0 and 255 degrees. Additionally, by allowing users to vary rotation intervals between two seconds and 2.75 hours, any DNA between 1 kb and 6 Mb can be separated.
During the course of a run, parameters such as electric field vectors, pulse length, and electric field strength can be changed. Also, a built-in platinum temperature sensor and flow-through valve permit the maintenance of temperatures within the buffer chamber. If the buffer temperature drifts beyond preset limits, the run is stopped ...
