New Tools Enable Gene Delivery

Electroporator Suppliers Lipid Transfection Reagents - Supplemental Table not in Print Edition Virus Transduction Reagents - Supplemental Table not in Print Edition Equibio Ltd.'s CelljecT Pro system Technologies that introduce DNA and RNA into eukaryotic cells, tissues, or organisms provide opportunities to study the regulation and function of genes and proteins. The biggest barrier that these technologies must overcome is the cell membrane, which is not permeable to highly charged macromol

Deborah Stull

| 7 min read

Listen with Speechify

0:00

7:00

Electroporator Suppliers

Lipid Transfection Reagents - Supplemental Table not in Print Edition

Virus Transduction Reagents - Supplemental Table not in Print Edition

Equibio Ltd.'s CelljecT Pro system

Technologies that introduce DNA and RNA into eukaryotic cells, tissues, or organisms provide opportunities to study the regulation and function of genes and proteins. The biggest barrier that these technologies must overcome is the cell membrane, which is not permeable to highly charged macromolecules such as DNA. Nevertheless, researchers have developed numerous approaches to overcome this challenge, including liposome-mediated and calcium phosphate (CaPO₄)-mediated transfection, electroporation, and viral transduction.

Each of these methods has advantages and disadvantages, often requiring researchers to compromise on one aspect of their experiment to maximize another. As a result, companies continue to improve existing technologies and to develop new ones that decrease the expense of transfection experiments, increase their efficiency, and enable transferring from one system or cell type ...

Interested in reading more?

Become a Member of

Receive full access to digital editions of The Scientist, as well as TS Digest, feature stories, more than 35 years of archives, and much more!

Join for free today

Already a member? Login Here