PCR on the Run

PCR Template Preparation Kits Epicentre Technologies' MasterAmp Buccal Swab DNA Extraction Kit Among the thousands of kits and products available to the life scientist, many provide a means of purifying genomic and plasmid DNA.1 Though most of these prepare highly purified DNA for specific applications such as Southern blotting, fluorescent automated sequencing, and RFLP analysis, some are designed to prepare DNA simply for use as PCR templates. Generally characterized as fast, easy, and eff

Written byMichael Brush

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PCR Template Preparation Kits

Epicentre Technologies' MasterAmp Buccal Swab DNA Extraction Kit

Among the thousands of kits and products available to the life scientist, many provide a means of purifying genomic and plasmid DNA.¹ Though most of these prepare highly purified DNA for specific applications such as Southern blotting, fluorescent automated sequencing, and RFLP analysis, some are designed to prepare DNA simply for use as PCR templates.

Generally characterized as fast, easy, and efficient, these template preparation products move away from the traditional DNA purification techniques including phenol and chloroform extractions and alcohol precipitations. DNA-releasing agents, paramagnetic beads, various resins, and spin columns are typical strategies employed here to quickly prepare PCR-ready DNA from a very broad range of sample materials.

The easiest and most direct way to prepare and amplify template DNA is to perform all the necessary steps within a single tube. Two products have been developed with ...

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