Probing Questions

Nucleic Acid Labeling Kits Nucleic Acid Labeling Kits (continued) Nucleic Acid Labeling Kits (continued) Genisphere's 3DNA™ Expression Array Detection Kit reagents The ability to label nucleic acids with a variety of tagged molecules has led to astonishing advances in molecular biology. Labeled DNA, RNA, and oligonucleotide probes have made possible techniques like the historic Southern blot, the northern blot, and related dot/slot-blotting procedures, in addition to in situ hybridizat

Michael Brush

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Nucleic Acid Labeling Kits

Nucleic Acid Labeling Kits (continued)

Genisphere's 3DNA™ Expression Array Detection Kit reagents

The ability to label nucleic acids with a variety of tagged molecules has led to astonishing advances in molecular biology. Labeled DNA, RNA, and oligonucleotide probes have made possible techniques like the historic Southern blot, the northern blot, and related dot/slot-blotting procedures, in addition to in situ hybridization studies and colony and plaque lifts. Recently, labeled cDNA probes have become an integral part of the gene expression analysis advances empowered by gene arrays. Beginning with the enzymatic incorporation of radioactivity and eventually employing nonisotopic alternatives, labeling methods and kits have evolved into a host of products and creative technologies that offer some powerful tools to the life scientist.

To label probes by nick translation, DNase I is used to create free 3' hydroxyl ends (nicks) within an unlabeled ...

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