Quantitative PCR (qPCR) is a commonly-used technique for quantifying nucleic acids. While the method appears simple on its surface, there are many steps where a researcher can introduce contaminants and other sources of error. By understanding which steps and reagents require careful optimization and validation, scientists can solve common qPCR challenges and perform reproducible experiments.

Download this article from Analytik Jena to learn about

  • Common challenges in qPCR workflows
  • How to overcome qPCR problems
  • Optimizing reaction conditions and using controls

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