Promega Imagine a world without 35S methionine. It's easy with Promega's TranscendTM Non-Radioactive Translation Detection Systems. Utilizing Promega's novel biotinylated lysine tRNA, these systems can be used to detect in vitro translation products after blotting with a variety of detectors, including Promega's own TranscenTM chemiluminescent substrates. No more wipe tests, no more radioactive waste disposal headaches. And results can be obtained in half the time.
One microliter of TNR translation reaction was spearated on a SDS-PAGE gel, blotted onto a PVDF membrane. The biotinylated proteins were detected with Streptavid-HRP and TranscendTM Chemiluminescent Substrates. (Lane 1 beta-galactosidase, Lanes 2 CAT and Lane 3 luciferase) Promega has developed the TranscendTM lysine tRNA, which has a biotin moiety linked to the e-amino group of a lysine molecule for use in any in vitro translation system. The biotin is linked to the lysine via a spacer arm to facilitate detection with avidin/strepavidin detectors and ...
