Quantitative real-time PCR (qPCR) is the tool of choice for quantifying nucleic acids in a variety of research fields ranging from agricultural biology to molecular diagnostics. Because factors such as sample quality, integrity, and purity influence experimental outcomes, researchers often rely on probe-based qPCR to increase the technology’s specificity and sensitivity. However, various probe types exist, each with their own pros and cons. Therefore, scientists must ensure they align probe technology and design with downstream applications to ensure qPCR success.
Download this ebook from LGC Biosearch Technologies to learn
- How to select the right probe based on sample characteristics
- Tips and tricks for matching probe design with downstream assays
- How to troubleshoot probe-based qPCRs
