Macrophages are critical immune cells that modulate the body’s response to infection, injury, inflammation, disease, and healing. Researchers differentiate and polarize cryopreserved monocytes into macrophage subtypes, then use live-cell imaging to validate their function. However, the phenotype and phagocytic ability of resulting macrophages can differ depending on the protocols used.
Download this article to explore a handy guide to optimizing macrophage polarization and functional assays for high-throughput workflows related to immunotoxicity and drug discovery studies.
