Melanoma cells stained with AIM melanoma (HMB45) strips Losing sight of the "big picture" concerns many researchers embroiled in running gels and performing assays. Visualizing a protein of interest in intact tissue sections can reconnect studies to the physiological realm and complement other types of data. However, immunohistochemical antigen-detection techniques can be daunting for the uninitiated. In October 1999, The Binding Site of San Diego, released the AIM (Antibody Impregnated Membrane
 Melanoma cells stained with AIM melanoma (HMB45) strips |
Dick Rowland, vice president of The Binding Site, notes, "Immunohistology is so complicated, involving optimization of antigen retrieval, primary and secondary antibody dilutions, and the development process. To get one antibody to work can be a long, time-consuming ...
When experiments refuse to cooperate, you try again and again. For Rafael Najmanovich, the setbacks ultimately pushed him in a new direction.
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