Transitioning to in-house real-time PCR methods for Mycoplasma detection can cut testing time from weeks to just a few hours. But for high-density advanced therapy medicinal products (ATMPs), standard protocols often fail. The large nucleic acid load from the cell product can interfere with extraction and amplification, reducing confidence in PCR-based detection and increasing the risk of false negatives.
This three-part compendium brings together PCR troubleshooting guidance with validation and optimization data, helping lab teams evaluate the technical and operational considerations behind in-house PCR-based Mycoplasma testing.
Access this compendium to explore
- PCR optimization tips for successful amplification
- Troubleshooting strategies for common PCR performance challenges
- How high-density samples can interfere with PCR-based detection
- Data from an in-house Mycoplasma testing workflow for cell therapy quality control (QC) release
