With conventional Western protocols, researchers can prepare one membrane per gel; duplicate filters can be prepared only by running duplicate gels. This approach can lead to blot-to-blot variability. In contrast, with the Multi-Replica Blotting Kit's proprietary transfer buffer and special blotting membranes, scientists can simultaneously make 10 blots from the same sample, reducing sample-to-sample variability. "There's a lot of confidence in being able to work with the same samples," observes Marcus.
The kit offers other benefits as well. First, it is a time-saver. The kit allows researchers to evaluate 10 proteins in less than three hours, instead of spending more than 24 hours to run and analyze 10 Westerns separately. Scientists also save money because they use less sample material and less of the reagents required to make and run the gels.
The key to blotting proteins from the gel to the 10-membrane stack is the transfer buffer's formulation. Marcus ...
