A Glowing Result

Induced cellular events distinguished by LumiTech's ApoGlow assay Apoptosis is an organized, energy-dependent process involving a number of well-defined pathways that lead to the death of a cell. Ultimately, these pathways converge on the mitochondria, causing changes in mitochondrial transmembrane potential that perturb cellular ADP:ATP ratios. Scientists at LumiTech Ltd. of Nottingham, United Kingdom, have shown that distinctive changes in the ADP:ATP ratio occur during cellular apoptosis and

Written byBarbara Cunningham

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Induced cellular events distinguished by LumiTech's ApoGlow assay

Apoptosis is an organized, energy-dependent process involving a number of well-defined pathways that lead to the death of a cell. Ultimately, these pathways converge on the mitochondria, causing changes in mitochondrial transmembrane potential that perturb cellular ADP:ATP ratios. Scientists at LumiTech Ltd. of Nottingham, United Kingdom, have shown that distinctive changes in the ADP:ATP ratio occur during cellular apoptosis and necrosis, enabling discrimination between these cellular states.¹ LumiTech's new ApoGlow kit uses bioluminescent detection of adenylate nucleotides to monitor changes in the cellular ratio of ADP:ATP. According to Kevin Slater, managing director at LumiTech, it is now possible to determine all possible results from putative cell viability-affecting agents within a single test.

In this system, cells are grown in 96-well microplates. Plates can be loaded directly from the incubator into the luminometer, which measures the intensity of light emitted following luciferase catalysis, ...

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