Articles by Thomas Unger

If you really think about it, data is only as good as the ability to acquire, quantitate, and compare various points that may be significant to the underlying observation. During the "historic" era of science most analyses were performed by visually evaluating a series of films or other forms of data and making side-by-side comparisons and interpretations. Conclusions were, for the most part, qualitative in nature, and rarely could true quantitative values be ascribed to particular bands or spo

For routine subcloning, nearly every manufacturer has a repertoire of standard laboratory strains. Many companies have expanded their range of products with special-function host strains that overcome many of the critical problems encountered during cloning and maintaining non-E. coli sequences in bacteria. Stratagene has a sizable entry in the special-use bacterial strain segment. Among some of the high-efficiency strains are those for maximizing or exploiting aspects of protein expression. Th

Comparison Chart N.A. Horn et al., Cancer Gene Therapy Using Plasmid DNA- Purification of DNA for Human Clinical Trials, Human Gene Therapy, 6: 565-573, 1995. R.M. Conry, A Carcinoembryonic Antigen Polynucleotide Vaccine for Human Clinical Use, Cancer Gene Therapy, 2: 33-38, 1995. Z. J. Ren et al., Cloning Of Linear DNAs In Vivo By Over- expressed T4 DNA Ligase Construction Of A T4 Phage Hoc Gene Display Vector. GENE, 195:303-311, 1997. M.V. Chudaev, S.A. Usanov, Expression Of Functionally Ac

Date: October 27, 1997 Comparison Chart Chart 2 The physiologic function of the immune system is simplistically viewed as a method to specifically respond and eliminate an initiating antigen. This process is mediated by a network of specialized cells and depends upon a pathway involving recognition, activation, differentiation and response of lymphocytes. This simplistic view is significantly more complicated when one is examining the biological nature of this response in greater detail. No

Date: September 29, 1997 Table Comparing Products Perhaps the most fundamental question when one has a nucleic acid or gene sequence in hand is "So, what does it do?" Whether these sequences encode a protein, or are promoter/enhancer regulatory elements, the general goal is to determine function and to understand the regulation and interaction of these genes and gene products with other cellular components. These questions are, of course, best addressed within a cellular context. The abilit