Courtesy of Nextgen Sciences

NextGen Sciences of Cambridge, UK, http://www.nextgensciences.com has released a baculovirus expression system that reduces the timeline of recombinant virus production by up to 10 days, according to company literature. Key to the technology is a modified viral vector, called flashBAC, that lacks part of a gene (ORF 1629) essential for virus replication in insect cells, says company spokesperson Mark Littlewood.

A bacterial artificial chromosome (BAC) sequence allows the vector to propagate in bacteria. Homologous recombination by cotransfection of flashBAC and a transfer vector in insect cells removes the BAC sequence and produces an intact viral DNA containing a restored ORF 1629 sequence and the gene of interest. Thus only viruses containing the gene of interest can replicate.

Other systems produce a mixture of recombinant and parental virus and require a lengthy plaque assay to screen for viruses containing the gene of interest,...

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