Bio-Rad Laboratories Inc. (NYSE: BIO and BIOb) today announced the launch of an assay website that allows droplet digital PCR (ddPCR™) users to locate existing assays or design new ones for mutation detection and copy number determination.
A critical aspect to the success of a ddPCR experiment is the use of a well-designed assay, which requires careful selection of PCR primers and probes specific to the gene of interest. Many factors influence assay design, such as amplicon length, GC content, sequence specificity, and melting temperature, all of which impact the final target sequence, primers, and probes. Flawed assay design can result in laborious testing and optimization, which may take several iterations.
Bio-Rad’s ddPCR assay website takes the guesswork out of ddPCR assay design and expands the portfolio of assays already available to ddPCR users. The website enables users to instantly design assays for any target of interest or order preexisting...
- Comprehensive analysis of assay specificity — perform sequence alignment to human, mouse, or rat reference genomes
- Multiple fluorophore options — choose either FAM or HEX probes for copy number determination assays
- Assay validation tools — order positive-control DNA for mutation detection assays