Bio-Rad Laboratories Inc. (NYSE: BIO and BIOb) today released the One-Step RT-ddPCR Advanced Kit for Probes which combines reverse transcription and Droplet Digital PCR (RT-ddPCR) for absolute RNA quantification. Delivering increased PCR efficiency, specificity, and sensitivity, this improved single-format reaction enables enrichment of rare target RNA sequences and detection of small differences in gene expression levels.
In the past, reverse transcription (RT) of RNA to complementary DNA (cDNA) required multiple extra steps prior to PCR amplification that could introduce experimental variability and the risk of sample cross-contamination. Bio-Rad introduced a single-reaction format to simplify and improve the throughput of RT-ddPCR experiments. Building on this product, the One-Step RT-ddPCR Advanced Kit offers an optimized enzyme and buffer composition along with protocol improvements that enable a greater range of quantification, detection, and applications.
For many standard runs, the advanced kit performs similarly to Bio-Rad’s original single reaction kit; however, for challenging RNA ...
