K.C. Cheng, D.S. Cahill, H. Kasai, et al., "8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G T and A C substitutions," Journal of Biological Chemistry, 267:166-72, 1992.
Keith C. Cheng (Division of Experimental Pathology, Penn State College of Medicine, Milton S. Hershey Medical Center, Hershey, Pa.): "Cellular DNA is damaged by oxygen free radicals generated during normal cellular respiration and phagocytosis as well as cell injury and exposure to environmental oxidants. That damage is mutagenic. Interest in a major product of that oxidative DNA damage, 8-hydroxyguanine (8ohG), has been heightened by its potential links to aging and human cancer. We determined the mutagenic potential of 8ohG in two complementary bacteriophage plaque color assays in which insertion of 8ohG into specified locations in test vectors was followed by replication in E. coli. The guanine analogue 8ohG engendered two types of mutation, both based upon mispairing with adenine; the type...
Interested in reading more?
Become a Member of
