Chromatrap®, a business unit of Porvair Sciences, has developed a new protocol that further extends the many advantages of its popular Chromatin Immunoprecipitation Sequencing (ChIP-seq) assay kits.
Key developments for the Chromatrap® ChIP-seq kit (version 1.2) include high quality chromatin can now be achieved via sonication or enzymatic digestion ; high and low abundant enrichment is possible from small chromatin samples ; improved antibody binding ; greater chromatin loading flexibility and the ability to use increased slurry volumes for difficult samples.
Chromatrap’s® ChIP-seq kit uses solid state technology in parallel with high throughput sequencing to deliver a streamlined ChIP-seq protocol from small cell numbers and low chromatin concentrations. Specifically adapted for broader chromatin concentrations, Chromatrap® ChIP-seq combines the dynamic range of Chromatrap® with the downstream analysis power of deep sequencing. This allows faster, more reproducible genome wide identification of TF binding sites and specific DNA associated protein modifications. With no ...
