Code Blue

Speed and sensitivity are the key features of GelCode® staining kits from Pierce Chemical Co. of Rockford, Ill. Designed to detect proteins in polyacrylamide gels, the kits include straightforward protocols and standardized reagents to speed up acquisition of data and increase reproducibility of results. "The common feature of the GelCode kits is that they are extremely user-friendly," says Linda Alger, a technical assistance manager with Pierce. In addition, the kits employ optimized chemis

Written byAlison Paladichuk
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The GelCode kit range includes four general protein-staining kits: Blue Stain, E-Zinc™ Reversible Stain, SilverSNAP™, and Color Silver Stain. The Blue Stain Kit offers one-step staining in one hour and can detect as little as 8 ng of protein. The remaining kits in this group can be used to detect subnanogram quantities of protein. Protein can be detected in only 15 minutes using the E-Zinc Reversible Stain Kit, and after an "eraser" step, can be eluted from the gel free of contamination. While SilverSNAP staining takes only 90 minutes, color silver staining for polychromatic staining of proteins takes considerably longer, but is the more sensitive of the two methods and is useful for distinguishing proteins of similar molecular weight.

Three kits for in-gel staining of proteins bearing specific moieties are new to the GelCode line: the 6xHis Protein Tag, Phosphoprotein, and Glycoprotein Staining Kits. Detection of such proteins usually depends ...

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