Cookbook For Eukaryotic Protein Expression: Yeast, Insect, and Plant Expression Systems

Date: November 9, 1998Baculovirus Expression Vectors In the recent past, efforts to elucidate the relationship between protein structure and biological function have intensified. Of particular interest is an understanding of the elements of sequence and structure that mediate specific functions. Often the protein of interest is in low abundance in its natural source and can be difficult to purify and/or unstable--subject to proteolytic cleavage or unfolding/non specific refolding during exhaustive purification. In other investigations, the examination of the involvement of specific residues in protein structure-function is hampered by the limitations of genetically modified proteins produced in bacterial systems. Consequently, a prerequisite to successful, detailed studies has, in many cases, necessitated the (over) production of biologically functional proteins. Modern advances in molecular biology and biochemistry can fulfill this need through the use of nonnative eukaryotic systems for the synthesis of recombinant proteins--that is, for the production of a "cloned" protein that ...