DNA Detection Without PCR; Sequence-Analysis Beerware; Shuffling the Genome Deck

Front Page | DNA Detection Without PCR; Sequence-Analysis Beerware; Shuffling the Genome Deck Courtesy of AcaClone Software SOFTWARE WATCH | Sequence-Analysis Beerware DNA sequence analysis software needn't be expensive. For a decade, molecular biologist Kjeld Olesen has spent much of his spare time developing pDRAW32, a free sequence-analysis software package for Windows PCs. Researchers can perform restriction enzyme analyses, edit sequences, create plasmid maps, cut and ligate "in silico

Written byBrendan Maher
| 3 min read

Register for free to listen to this article
Listen with Speechify
0:00
3:00
Share

SOFTWARE WATCH | Sequence-Analysis Beerware

DNA sequence analysis software needn't be expensive. For a decade, molecular biologist Kjeld Olesen has spent much of his spare time developing pDRAW32, a free sequence-analysis software package for Windows PCs. Researchers can perform restriction enzyme analyses, edit sequences, create plasmid maps, cut and ligate "in silico," analyze GC content, make homology plots, and select primer sites from a user-specified database. Now in version 1.1.60, pDRAW32 (www.acaclone.com) even has a feature that creates a virtual gel. "It's designed to assist the molecular biologist in what he does when he's working in the laboratory."

Olesen says he started developing pDRAW32 because 10 years ago, the only available applications had to be accessed through remote servers using UNIX. Though he's unsure how many researchers use the software, Olesen says his Web site and its mirrors have logged more than 100,000 visitors. He continues to update the program ...

Interested in reading more?

Become a Member of

The Scientist Logo
Receive full access to digital editions of The Scientist, as well as TS Digest, feature stories, more than 35 years of archives, and much more!
Already a member? Login Here

Meet the Author

Published In

Share
Image of a woman with her hands across her stomach. She has a look of discomfort on her face. There is a blown up image of her stomach next to her and it has colorful butterflies and gut bacteria all swarming within the gut.
November 2025, Issue 1

Why Do We Feel Butterflies in the Stomach?

These fluttering sensations are the brain’s reaction to certain emotions, which can be amplified or soothed by the gut’s own “bugs".

View this Issue
Olga Anczukow and Ryan Englander discuss how transcriptome splicing affects immune system function in lung cancer.

Long-Read RNA Sequencing Reveals a Regulatory Role for Splicing in Immunotherapy Responses

Pacific Biosciences logo
Research Roundtable: The Evolving World of Spatial Biology

Research Roundtable: The Evolving World of Spatial Biology

Conceptual cartoon image of gene editing technology

Exploring the State of the Art in Gene Editing Techniques

Bio-Rad
Conceptual image of a doctor holding a brain puzzle, representing Alzheimer's disease diagnosis.

Simplifying Early Alzheimer’s Disease Diagnosis with Blood Testing

fujirebio logo

Products

Eppendorf Logo

Research on rewiring neural circuit in fruit flies wins 2025 Eppendorf & Science Prize

Evident Logo

EVIDENT's New FLUOVIEW FV5000 Redefines the Boundaries of Confocal and Multiphoton Imaging

Evident Logo

EVIDENT Launches Sixth Annual Image of the Year Contest

10x Genomics Logo

10x Genomics Launches the Next Generation of Chromium Flex to Empower Scientists to Massively Scale Single Cell Research