Let It Shine

Fluorescence Detection Products Courtesy of BD PharMingenMulticolor immunofluorescent cell staining showing cell surface FITC (green) staining and intracellular PE (orange) and APC (red) staining. Is radioactive detection of biomolecules destined to the same fate as the Berlin Wall? The multitude of nonisotopic detection methods now available suggests that the 21st century may come to be billed as the "nonisotopic research century." The broadest common denominator of this up and coming cast of

Written byJorge Cortese

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Fluorescence Detection Products

Courtesy of BD PharMingen

Multicolor immunofluorescent cell staining showing cell surface FITC (green) staining and intracellular PE (orange) and APC (red) staining.

Is radioactive detection of biomolecules destined to the same fate as the Berlin Wall? The multitude of nonisotopic detection methods now available suggests that the 21st century may come to be billed as the "nonisotopic research century." The broadest common denominator of this up and coming cast of nonisotopic technologies is fluorescence detection. Throughout this year, LabConsumer will review many specialized fluorescence techniques for detection of molecules on surfaces and in solution.

Fluorescence technology tries at times to be all things to all people, and the researcher usually must make a complex choice about protein detection. To help with the first--and difficult--step of selecting an available assay or deciding to design one, the general avenues of fluorescence-based protein detection technologies will be explored. At the very ...

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