Steps in positional cloning. Positioning of disease loci to chromosomal regions requires multidisciplinary collaborations.
"You have a clinician out in Israel gathering patients, a medical geneticist doing genetic mapping, my lab doing physical mapping, and Washington University sequencing. All these things come together and, boom, in record time you have this gene," says Green.

Green goes on to point out that this is a classic example of finding a gene from sequences the Human Genome Project is posting on the World Wide Web every night ( Other groups trying to find PDS were looking at the Washington University sequence as well.3 "It was a horse race. We competed to the end," says Green.

Going Beyond the Gene

Once researchers located PDS, they used the sequence of nucleotides to identify a protein the scientists named pendrin. A computer search found the amino acid sequence of pendrin is...

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