To perform matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS), researchers combine the sample with a molar excess of matrix—usually an aromatic organic acid that absorbs light energy at the laser's wavelength—and spot this mixture onto a metal target plate. When the focused light pulse strikes the target, it rapidly excites the matrix, inducing ionization and vaporization of both the matrix and the sample molecules in the surface layer. The desorbed ions are accelerated in a vacuum through an electric field into the TOF mass analyzer, where they travel toward the detector at a constant velocity characteristic of their mass-to-charge ratio (m/z). Some instruments are designed with a reflectron, an ion "mirror" that extends the length of the flight path and increases resolution. The m/z normally represents the mass of the ion, assuming each ion has a single charge. This "soft" ionization process produces very little fragmentation and enables scientists ...
Negative News
Manchester, UK-based Micromass has added negative-ion detection capabilities to its M@LDI™ family of mass spectrometers. The company unveiled this new feature in New Orleans at Pittcon 2002, an annual trade fair where more than 30,000 conferees and exhibitors in virtually every laboratory discipline launch new products. To perform matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS), researchers combine the sample with a molar excess of matrix̵
Written byHillary Sussman
| 2 min read
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