from Stratagene Signal transduction seems like such a simple concept: transmitting messages within and between cells. But when you consider the hundreds of genes that are involved in signal transduction-receptors, kinases, phosphatases, to mention but a few-achieving an understanding seems like an onerous task. To make matters worse, what these signaling molecules do in a test tube, more often than not, is not what they do in a living cell. Stratagene's novel reporting systems for signal transduction pathways, PathDetect in Vivo Signal Transduction Pathway trans-Reporting Systems, offer new insights into the process by providing the opportunity to detect the activation of several signal transduction pathways in vivo, using a dual reporting/activating system.
The pFR-Luc plasmid was cotransfected into the HeLa cells with the JNK activator plasmid (panel A), the Elk-1 activator plasmid (panel B) and the CREB activator plasmid (Panel C). Panel A shows a greater than 100x activation of ...
