Imagine cloning a gene into several different expression vectors without restriction enzymes or ligations. Invitrogen's Echo Cloning System promises just that. Based on the Univector Plasmid-fusion System developed by Steve Elledge at Baylor College of Medicine, the Echo System avoids having to PCR amplify and sequence an insert every time it is subcloned into a new vector.1 The newly released Echo System combines Invitrogen's proven TOPO® Cloning System for five-minute cloning of PCR products with the Univector System to rapidly transfer clones into several different protein expression vectors.
Invitrogen's Echo Cloning System The Echo System is composed of a donor and acceptor vector that are recombined into a single protein expression vector by Cre-lox-mediated site-specific recombination. LoxP and loxH elements are 34 bp sequences that are recognized by the 35 kDa Cre recombinase. Binding by Cre recombinase brings together the lox sequences on the donor and acceptor plasmids and catalyzes ...
