Cell or tissue lysates for use with RayBio® ELISA kits can be prepared using most conventional methods, e.g. homogenization of cell or tissue in RayBio® Lysis Buffer. You may also use your own lysis buffer, such as RIPA or other formulations optimized for immunoprecipitation.
We strongly recommend adding a protease inhibitor cocktail to the lysis buffer prior to homogenization. Most general biochemical supply companies including Roche, Sigma-Aldrich, Pierce, and Calbiochem stock a wide variety of these products. Since susceptibility to proteolytic cleavage and the type of proteases present in the lysate vary, we do not recommend a specific product. Instead, your choice of which combination of protease inhibitors to use should be based upon a literature search for your protein(s) of interest and/or tissue or cell type. Phosphatase inhibitors may be used but are not necessary unless the antibodies used in the kit specifically recognize phosphorylated forms of the protein.
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