Consistency may be the hobgoblin of small minds, but it is absolutely essential to studies requiring electrophoretic separations of proteins. Consistency, high resolution, and stability are all important when trying to determine levels of protein expression, for example, or when documenting a purification scheme of a protein. Novex's latest offering of precast polyacrylamide gels, the NuPAGE Neutral pH Electrophoresis System, provides advantages in all these areas.
Western blot of proteins synthesized in an E. coli expression system, showing the separation of a 22.5 and 23.5 kD protein. (courtesy of Farah Natoli, Praecia Pharmaceuticals, Inc. The neutral operating buffer prevents protein modification during electrophoresis, leaving samples unchanged and resulting in sharper bands. The pH also carries over into the transfer process, which allows for more efficient transfers in Western blotting protocols. The gels have an increased shelf life, (gels can be used up to one year after purchase) because of the ...
