New England Biolabs (NEB®) announced today that they have released a novel, thermostable reverse transcriptase, WarmStart® RTx Reverse Transcriptase, specifically designed for nucleic acid detection.
Sir Richard Roberts, Chief Scientific Officer at NEB, commented on the announcement, “NEB has a 40-year history innovating in the field of molecular biology enzymes and related technologies. WarmStart RTx is an in silico-engineered thermostable reverse transcriptase, designed by our researchers for speed, sensitivity and inhibitor resistance in reverse transcription loop-mediated isothermal amplification (RT-LAMP), RT-PCR, and other applications where the detection of low-quantities of target RNA from complex sample matrices is critical”.
WarmStart RTx is reversibly inhibited at room temperature, and is activated when warmed above 40 degrees C, enabling researchers to set up their reactions at room temperature with no background reverse transcriptase activity. Maximum activity is reached at 55 degrees C, enabling high-temperature cDNA synthesis. This results in more consistent target detection across ...
