Tyrosine Phosphorylantion State-Sensitive Antibodies

Protein tyrosine kinases (PTKs) are a diverse lot, including both transmembrane and soluble cytoplasmic enzymes. Roughly 10 percent of a cell's proteins are subject to covalent modification via phosphorylation, but tyrosine (Tyr) residues are modified in only about one out of every 100 protein phosphorylation events. When Tyr-phosphorylation occurs, cells pay heed. PTKs play vital roles in a wide variety of cellular processes, including growth, differentiation, and metabolism.

Immunohistochemical staining with mAb 4G10. Image provided by Upstate Biotechnology.

If you are in the business of detecting, visualizing, or purifying Tyr-phosphorylated proteins, then antiphosphotyrosine antibodies [ anti-(P)Tyrs] will definitely be on your shopping list. Several types of anti-(P)Tyrs are commercially available. Polyclonal preparations are sold by various vendors, and a number of different monoclonal anti (P)Tyrs are also on the market. Most of the currently available anti-(P)Tyrs bind free (P)Tyr quite well--with affinities in the µM range...

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