ZEISS is introducing LSM 880 with Airyscan. The new confocal laser scanning microscope offers high sensitivity, enhanced resolution in x, y and z, and high image-acquisition speed in one system.
Sensitivity, Resolution, and Speed
Surveys results reveal that confocal imaging would benefit the most from increased sensitivity, resolution, and speed. Airyscan extends exactly those parameters. Users achieve a 1.7× higher resolution in all spatial dimensions, 140 nm laterally and 400 nm axially. The improved sensitivity leads to better image quality and increased speed. The whole imaging process is possible with standard sample preparation and labeling protocols.
The Airyscan PrincipleA classic confocal microscope illuminates one spot on the sample to detect the emitted fluorescence signal. Out-of-focus emission light is rejected at a pinhole, the size of which determines how much of the Airy disk reaches the detector. Users can increase the resolution by making the pinhole smaller, but the signal-to-noise ratio drops ...
