What is it? Acrydite is a molecular chimera containing an acrylic group and phosphoramidite that can be used as a platform for oligo construction (via the phosphoramidite part) and as a vehicle to attach oligos to surfaces (via the acrylic portion). What makes this particular molecule so useful is that it attaches to surfaces in a single step--and a number of different surfaces are possible--forms a bond that is resistant to high temperatures, and leaves the attached oligo accessible for hybridization assays that are both rapid and efficient.
Five layer gel with horizontal capture layers A-E. The five samples of fluorescently labeled oligonucleotides are each complementary to only one of the five capture probes. At left, a mixture of all five are loaded in the same well. How is it used? One use of this technology that has a range of applications is gel-based hybridization assays. Oligos made with acrydite ...
