A Shot In The Dark: Products For The Chemiluminescent Detection Of DNA

Date: May 11, 1998 Author: Deborah A. Wilkinson Products for the Chemiluminescent Detection of DNA t's late at night. You've added radiolabeled probe to your Southern blot and just placed it in the incubator. Time to go home! But not quite. Now you have to clean up the radioactive waste that oozed out while you were sealing the bag. "There has to be a better way," you think. Well, you might want to consider one of the nonradioactive, chemiluminescent detection systems. The high backgrounds and low sensitivities associated with earlier generations of nonisotopic detection systems left many researchers frustrated with these techniques. However, significant improvements have been made in chemiluminescent detection methods during the past few years. These systems now merit a closer look. Recently developed chemiluminescent substrates have detection sensitivities that rival those of radioactive methods. Another advantage of chemiluminescent detection systems is that the probes are stable, at ...