Researchers need precise tools to measure molecular interactions and binding affinities for basic research and drug discovery. Conventional approaches such as isothermal calorimetry (ITC) present challenges, including large sample volume requirements, slow and difficult to optimize assays, and complicated parsing of binary and ternary interactions. Spectral Shift assays using the Dianthus plate-based affinity screening platform enable scientists to determine dissociation constants at higher throughput, in solution, while using only minimal amounts of sample.

Download this poster from NanoTemper to learn how scientists applied Spectral Shift assays using Dianthus to assess and optimize the binding affinity of synthetic degraders targeting the oncogene regulator WDR5.

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