The AntibodyArray is a membrane on which antibodies to a variety of protein ligands are immobilized. Users of the technology apply a cell lysate to the array and incubate to allow proteins to bind to their respective antibodies. They then detect the protein of interest by exposing the membrane to an antibody against that protein. The identity of proteins with which the target protein interacts is determined by the target protein's position on the membrane.
Ernest Lee, chair and professor of biochemistry and molecular biology at the New York Medical College, uses Hypromatrix's AntibodyArrays in his research, with a slight modification to the usual assay procedure. Lee's group tags their protein of interest with digoxigenin (DIG), exposes the antibody array screen to a cell lysate, and then exposes the array to the DIG-labeled protein. The labeled protein will stick to any protein on the array that contains a binding site ...
